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Molecular Basis of Inheritance

Transcription

Transcription is the cell copying one gene from DNA into a strand of RNA. It is how the instructions in a gene leave the safe DNA and become a working message.

An enzyme, RNA polymerase, binds the start of a gene, unwinds a short stretch of DNA, and reads one strand. It builds an RNA copy using base pairing, but with one change: RNA uses uracil (U) in place of thymine (T), so it pairs U with A.

The finished messenger RNA carries the gene's message out to the ribosomes, where it is read to build a protein. Only one strand of the DNA is read, called the template strand.

Where students slip up

Only one DNA strand is read as the template, not both. And RNA uses U instead of T. Students often copy the DNA letter for letter and forget the T to U swap, or try to read both strands at once. One strand, and every A on it becomes U on the RNA.

Hold onto this

One gene, one strand read, T becomes U. That is transcription.

Why it helps in the exam: the template versus coding strand, and the U-for-T rule, are classic traps.

Where this connects

The RNA made here is not the end. It travels to the ribosome to be read into a protein, so transcription and translation are two halves of one flow: DNA to RNA to protein.

The next step: translation

Quick check

During transcription, the RNA base that pairs with adenine (A) on the DNA template is:

  • AThymine (T)
  • BUracil (U)
  • CGuanine (G)
  • DCytosine (C)